Laboratory Diagnosis: Molecular Techniques (FOCUS, Volume 4.4)

Fee:None
Length: 40 minutes
Description:This issue of FOCUS takes a closer look at the molecular techniques most commonly used in public health: polymerase chain reaction (PCR), pulsed field gel electrophoresis (PFGE), and ribotyping.
To Access and Complete This Training:

To create a login ID and password for the NCIPH Training Website, click on the Create An Account link. If you have previously created an account, click on the Login to Training Link. Please read over the information on this page first.

This training was developed with the support of the UNC Preparedness and Emergency Response Learning Center (UNC PERLC), a funded project of the Centers for Disease Control and Prevention (CDC Cooperative Agreement 1U90TP000415).
PERLC

Learning Objectives

Training Personnel

Authors:

Amy Nelson, PhD, MPH

Lauren N. Bradley, MHS

FOCUS Workgroup

The author(s) and reviewer(s) of this training have no personal financial relationships with commercial interests relevant to this presentation to disclose. Author, narrator, reviewer affiliations listed were current at the time of training development.

Competencies and Capability Functions Addressed

This training addresses selected applied epidemiology, core public health, and public health preparedness and response competencies and public health preparedness capability functions. (Please note: The competencies included on this site are just a few of the public health competencies which have been established. Training participants may find alignment between this training and other competency sets not included on this site.)

Public Health Preparedness & Response Core Competencies
2.5. Manage the recording and/or transcription of data according to protocol.

References

Johnson DW, Pieniazek NJ, Griffin DW, Misener L, Rose JB. Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Appl Environ Microbiol. 1995;61:3849-3855.

Centers for Disease Control and Prevention. Multistate outbreak of Escherichia coli O157:H7 infections associated with eating ground beef --- United States, June--July 2002. MMWR Morb Mort Wkly Rep. 2002;51:637-639. Available at: http://www.cdc.gov/mmwr/preview/mmwrhtml/mm5129a1.htm. Accessed November 30, 2006.

3. Fontana J, Stout A, Bolstorff B, Timperi R. Automated ribotyping and pulsed field electrophoresis for rapid identification of multidrug-resistant Salmonellas Serotype Newport. Emerg Infect Dis [serial online]. 2003;9:496-499. Available at: http://www.cdc.gov/ncidod/EID/vol9no4/02-0423.htm. Accessed December 14, 2006.

To Access and Complete This Training:

To create a login ID and password for the NCIPH Training Website, click on the Create An Account link. If you have previously created an account, click on the Login to Training Link. Please read over the information on this page first.